Coding

Part:BBa_K2729011:Design

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-29)


DENV2 ancC-prM-E (Dengue Virus Serotype II)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 946
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1397
    Illegal AgeI site found at 1108
    Illegal AgeI site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 349


Design Notes

Since the genes are separated from non-structural region, the proteins and the replicated genes are non-infectious and not reproduced after the first infection.


Source

Since the genes consist of dengue virus structural region, they are derived from dengue virus.

References